Pesticide residues in human blood are a critical concern due to their potential health risks. This study presents the development and validation of a novel assay utilizing Gas Chromatography-Mass Spectrometry (GC/MS) for detecting pesticide residues in human blood. The assay aims to enhance the accuracy, sensitivity, and reliability of pesticide residue analysis, addressing gaps in current detection methods. The assay development involved optimizing sample preparation techniques, including blood extraction and clean-up processes, to ensure minimal interference and maximum recovery of pesticide residues. We employed GC/MS to separate and identify a wide range of pesticides, including organophosphates, carbamates, and organochlorines, based on their unique mass spectra and chromatographic profiles.

Validation of the assay was conducted according to established guidelines, including assessment of specificity, sensitivity, linearity, and reproducibility. The method demonstrated high specificity with no significant cross-interference from other compounds. Sensitivity was validated through detection limits well below regulatory thresholds, and linearity was confirmed across a broad concentration range. Reproducibility was ensured with consistent results across multiple runs and different operators. The developed assay provides a robust and reliable tool for the detection and quantification of pesticide residues in human blood, offering improved analytical capabilities compared to existing methods. This advancement is crucial for monitoring pesticide exposure, assessing health risks, and supporting regulatory compliance. The study underscores the importance of accurate pesticide residue analysis in safeguarding public health and contributes to the broader field of environmental and clinical toxicology.

Pesticide residues, human blood, assay development

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