Articles | Open Access |

A study of biochemical profiles and antioxidant potential in fresh apple genotypes

Zane Collins , Department of Food Science and Human Nutrition, Food Sciences Building, Iowa State University, Ames, IA 50010, USA
Knox Harrison , Department of Food Science and Human Nutrition, Food Sciences Building, Iowa State University, Ames, IA 50010, USA

Abstract

Apple (Malus domestica) is a widely consumed fruit, known for its nutritional and health benefits. This study investigates the biochemical profile and antioxidant activity of fresh fruits from different apple genotypes, focusing on key components such as sugars, organic acids, phenolic compounds, and antioxidant capacity. A total of five apple genotypes (G1, G2, G3, G4, and G5) were selected, and the fruit samples were analyzed for their biochemical composition using high-performance liquid chromatography (HPLC) and spectrophotometric methods. The results showed significant variability in sugar content, with fructose and glucose being the predominant sugars. Organic acids, such as malic acid, were found in varying concentrations across genotypes. Phenolic compounds, including flavonoids and phenolic acids, demonstrated antioxidant activity, which was highest in Genotype G3. The study highlights the genetic variation in apple fruits that affects their nutritional composition and antioxidant potential, providing valuable information for breeding programs aimed at enhancing fruit quality.

Keywords

Biochemical Profiles, Antioxidant Potential, Apple Genotypes

References

Hyson, D.A. A comprehensive review of apples and apple components and their relationship to human health. Adv. Nutr. 2011, 2, 408–420. [Google Scholar] [CrossRef] [PubMed]

Mureșan, A.E.; Sestras, A.F.; Militaru, M.; Păucean, A.; Tanislav, A.E.; Pușcaș, A.; Sestras, R.E. Chemometric comparison and classification of 22 apple genotypes based on texture analysis and physico-chemical quality attributes. Horticulturae 2022, 8, 64. [Google Scholar] [CrossRef]

Fotirić Akšić, M.; Nešović, M.; Ćirić, I.; Tešić, Ž.; Pezo, L.; Tosti, T.; Meland, M. Polyphenolics and chemical profiles of domestic Norwegian apple (Malus × domestica Borkh.) cultivars. Front. Nutr. 2022, 9, 941487. [Google Scholar] [CrossRef] [PubMed]

Arnold, M.; Gramza-Michalowska, A. Recent development on the chemical composition and phenolic extraction methods of apple (Malus domestica)—a review. Food Bioprocess Technol. 2024, 17, 2519–2560. [Google Scholar] [CrossRef]

da Silva, L.C.; Viganó, J.; de Souza Mesquita, L.M.; Dias, A.L.B.; de Souza, M.C.; Sanches, V.L.; Chaves, J.O.; Pizani, R.S.; Contieri, L.S.; Rostagno, M.A. Recent advances and trends in extraction techniques to recover polyphenols compounds from apple by-products. Food Chem. X 2021, 12, 100133. [Google Scholar] [CrossRef]

Kalinowska, M.; Bielawska, A.; Lewandowska-Siwkiewicz, H.; Priebe, W.; Lewandowski, W. Apples: Content of phenolic compounds vs. variety, part of apple and cultivation model, extraction of phenolic compounds, biological properties. Plant Physiol. Biochem. 2014, 84, 169–188. [Google Scholar] [CrossRef]

Geană, E.-I.; Ciucure, C.T.; Ionete, R.E.; Ciocârlan, A.; Aricu, A.; Ficai, A.; Andronescu, E. Profiling of phenolic compounds and triterpene acids of twelve apple (Malus domestica Borkh.) cultivars. Foods 2021, 10, 267. [Google Scholar] [CrossRef]

Jakobek, L.; Matić, P. Phenolic compounds from apples: From natural fruits to the beneficial effects in the digestive system. Molecules 2024, 29, 568. [Google Scholar] [CrossRef]

Hassanpour, S.; Maherisis, N.; Eshratkhah, B.; Baghbani Mehmandar, F. Plants and secondary metabolites (tannins): A review. Int. J. For. Soil Eros. 2011, 1, 47–53. [Google Scholar]

Lees, G.L.; Suttill, N.H.; Wall, K.M.; Beveridge, T.H. Localization of condensed tannins in apple fruit peel, pulp, and seeds. Can. J. Bot. 1995, 73, 1897–1904. [Google Scholar] [CrossRef]

Minocha, S.; Kumari, S.; Tiwari, A.; Gupta, A.K. An overview on tannins. Int. J. Pharm. Biol. Sci. Arch. 2015, 3, 1–3. [Google Scholar]

Min, B.R.; Barry, T.N.; Attwood, G.T.; McNabb, W.C. The effect of condensed tannins on the nutrition and health of ruminants fed fresh temperate forages: A review. Anim. Feed Sci. Technol. 2003, 105, 3–19. [Google Scholar] [CrossRef]

McMahon, L.R.; Leon, F.; McAllister, T.; McAllister, T.A.; Berg, B.P.; Majak, W.; Acharya, S.N.; Popp, J.D.; Jürgen Popp, J.P.; Coulman, B.E.; et al. A review of the effects of forage condensed tannins on ruminal fermentation and bloat in grazing cattle. Can. J. Plant Sci. 2000, 80, 469–485. [Google Scholar] [CrossRef]

Wojdyło, A.; Nowicka, P.; Turkiewicz, I.P.; Tkacz, K.; Hernandez, F. Comparison of bioactive compounds and health promoting properties of fruits and leaves of apple, pear and quince. Sci. Rep. 2021, 11, 20253. Available online: https://www.nature.com/articles/s41598-021-99293-x.pdf (accessed on 15 December 2024). [CrossRef]

Yang, S.; Meng, Z.; Li, Y.; Chen, R.; Yang, Y.; Zhao, Z. Evaluation of physiological characteristics, soluble sugars, organic acids and volatile compounds in ‘Orin’ apples (Malus domestica) at different ripening stages. Molecules 2021, 26, 807. [Google Scholar] [CrossRef]

Cirillo, A.; Spadafora, N.D.; James-Knight, L.; Ludlow, R.A.; Müller, C.T.; De Luca, L.; Di Vaio, C. Comparison of volatile organic compounds, quality, and nutritional parameters from local Italian and international apple cultivars. Horticulturae 2024, 10, 863. [Google Scholar] [CrossRef]

Mignard, P.; Beguería, S.; Giménez, R.; Font i Forcada, C.; Reig, G.; Moreno, M.Á. Effect of genetics and climate on apple sugars and organic acids profiles. Agronomy 2022, 12, 827. [Google Scholar] [CrossRef]

Lee, K.W.; Kim, Y.J.; Dae-Ok, K.; Lee, H.J.; Lee, C.Y. Major phenolics in apple and their contribution to the total antioxidant capacity. J. Agric. Food Chem. 2003, 51, 6516–6520. [Google Scholar] [CrossRef]

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Zane Collins, & Knox Harrison. (2025). A study of biochemical profiles and antioxidant potential in fresh apple genotypes. The American Journal of Applied Sciences, 7(03), 1–5. Retrieved from https://theamericanjournals.com/index.php/tajas/article/view/5909