TECHNOLOGY FOR IN VITRO REARING OF PARASITICS
Khabiba Khidoyatova , Researcher, Vice Dean - Tashkent State University of Economics, Uzbekistan Rasul Jumaev , Professor, Doctor of Agricultural Sciences, Tashkent State Agrarian University, UzbekistanAbstract
The technology of in vitro propagation of parasitic entomophages is a very important process. This direction is very important for the agricultural economy. An average of 1.8-2.2 million dollars is spent on the propagation of parasitic entomophages in our country per year. If we introduce the technology of in vitro propagation of parasitic entomophages, this will reduce costs by 2-3 times. Propagating parasitic organisms in this way will save significant economic costs.
The studies on in vitro rearing Hymenoptera have been conducted successfully in Uzbekistan. For these parasites, cooking and the three main types of media were made from the hemolymph of the wax moth (G.melonnella) and cotton worm Helicoverpa armigera (Heliothis armigera), egg yolk, natural milk 10%. Mass production of in vitro reared Braconidae and Trichogrammatidae its utilization in the fields showed good effectiveness in controlling agricultural economy.
Keywords
Agricultural economy, artificial media, parasitoid growth factors
References
R.А.Jumaev. In vitro rearing of Trichogramma (Hymenoptera: Trichogrammatidae) European science review. – 2016. - № 9 – 10. - pp. 11–13. (In Vienna).
Jumaev RA, Kimsanboev XX, Adilov MM, Rustamov AA, The technology of rearing Braconidae in vitro in biolaboratory, European Science Review 3-4, 3-5 (2017).
Jumaev R, Invitro rearing of parasitoids, E3S Web of Conferences 371, 01032 (2023).
Jumaev R, Sobirov TR, Azimova MB, Modern application and decoration art of Bukhara, Academicia: An International Multidisciplinary Research Journal 111, 117-124 (2021)
Li Li-ying, Liu Wenhui. Rearing Trichogramma spp, with artificial diets, containing hemolymph of different insects. Parasitoids and predators (insecta) of agricultural and forestry arthropod pests -1997. -pp 335-337. (In china)
Li Li-ying, Liu Wenhui, etc. In vitro rearing of Trichogramma spp and Anastatus sp in artificial “eggs” and the methods of mass production. Parasitoids and predators (insecta) of agricultural and forestry arthropod pests.-1997. –pp.344-357.(In china).
Кимсанбаев. Х.Х., Жумаев Р.А. Renewing and rearing technology of Brаcon hebetor Say in Biolaboratory; Материалы VIII-оймеждународной научно - практической конфе-ренции молодых исследователей, г. Волгоград, 2014. –pp.257-259.
Huang Xin. Study on the use of Habrobracon hebetor. Bulletin of Biological Control 2 (2): -1986. –pp. 70 – 75 (In Chinese).
Xie Zhong-neng, etc. In vitro culture of Habrobracon hebetor (Say) (Hym.,Braconidae).Chines J.of Biological Control // 1989. - № 5(2): –pp. 49 – 51. (In China).
Xie Zhong-neng, etc. In vitro culture of the ectoparasitoid Bracon greeni Ashmead. Acta Entomologica Sinica // 1989. - № 32(4): –pp. 433 – 437. (In China).
Lebedeva N, Akhmedova Z, Kholmatov B, Revision of stoneflies insecta: plecoptera fauna in Uzbekistan, E3S Web of Conferences 258, 08030 (2021)
Axmatovich JR, In vitro rearing of trichogramma Hymenoptera: Trichogrammatidae, European science review 9-10, 11-13 (2016) 8.
Abdushukirovich SB, Xamraqulovich KX, Axmatovich JR, Karimbaevich SS, Rearing of Trichogramma species T evanescens T pintoi T chilonis in vitro culture, European science review 1-2, 29-31 (2018)
Axmatovich JR, Karimbaevich SS, Qizi NB, O'g'li BSS, Bioecology of generations of Trichogramma diluted by different methods, European science review 3-4, 25-28 (2018)
Rustamovich SI, Xamrakulovich KX, Axmatovich RA, Nozimxon J, Axmatovich JR, Bioecology harm of tobacco trips for the cotton plant and measure of counteraction, European science review 3-4, 29-31 (2018)
Suyunbayev S, Khusenov U, Khudayberganov S, Jumayev S, Kayumov S, Improving use of shunting locomotives based on changes in infrastructure of railway station, E3S Web of Conferences 365, 05011 (2023)
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