Articles | Open Access | DOI: https://doi.org/10.37547/tajabe/Volume04Issue03-01

EFFECTS OF GRAPHENE OXIDE NANOSHEET ON GERMINATION AND SEEDLING ATTRIBUTES ON TAGETES ERECTA L. AND CALENDULA OFFICINALIS L.

Kunal Adhikary , Ph.D Research scholar, Department of Floriculture and Landscape Architecture, Faculty of Horticulture. Bidhan Chandra Krishi Vishwavidyalaya, Mohanpur- 741252, Nadia (WB), India
Tapas Mandal , Department of Department of Floriculture and Landscape Architecture, Faculty of Horticulture, Bidhan Chandra Krishi Vishwavidyalaya, Mohanpur- 741252, Nadia (WB), India
Jayoti Majumder , Assistant professor, Department of Department of Floriculture and Landscape Architecture, Faculty of Horticulture, Bidhan Chandra Krishi Vishwavidyalaya, Mohanpur- 741252, Nadia (WB), India
Prasenjit Mandal , Ph.D Research scholar, Department of Chemistry, University of Kalyani- 741235, Nadia (WB), India

Abstract

In recent times, nanotechnology is a branch of precision farming and sustainable agriculture where it expresses all sides of development for better utilization of resources in agriculture. Graphene oxide (GO) nanosheet is a two-dimensional crystal structure formed by a flat monolayer of sp² hybridized carbon atoms arranged in a hexagonal arrangement. Marigold (Tagetes erecta L.) and Calendula (Calendula officinalis L.) are commercially explored flower crops of the family Asteraceae. These flower species contain a sufficient amount of active compounds, used in various commercial sectors. Graphene oxide nanosheets were produced by the commercial chemical method.  Characterization of GO is done on powder X-ray diffraction (XRD), Fourier transforms infrared (FTIR) & Scanning electron microscopy (SEM). Seeds were collected from recognized sources and after surface sterilization; seeds were treated with GO nano solution with different concentrations (0, 20,40,60,80,100,120 and 140mg/l respectively). The conducted experiment found that GO in the concentration range from 40 to 80 mg/l (T2 to T4) gives positive results in the maximum of all aspects in both the annuals. The maximum root length of the marigold was recorded in T2 (5.91cm) and shoot length in T5 with 4.63cm. The coefficient of the velocity of germination (CVG) for calendula was observed maximum in T3 (32.17) and in marigold T2 (46.34).  In GO treated seeds were having a dense rooting system with good seedling growth, early germination rate also observed in treated seeds in comparison to control.

Keywords

GO, Germination, Growth, FTIR

References

Allen, S.J., Watson, J.J., Shoemark, D.K., Barua, N.U., & Patel, N.K. (2013). GDNF, NGF and BDNF as therapeutic options for neurodegeneration. Pharmacology & Therapeutics, 138, 155–175. doi: 10.1016/j.pharmthera.2013.01.004

Begum, P., Ikhtiari, R., Fugetsu, B., Matsuoka, M., Akasaka, T., & Watari, F. (2012). Phytotoxicity of multi-walled carbon nanotubes assessed by selected plant species in the seedling stage. Applied Surface Science ,262,120–124. doi: 10.3390/nano4020203

Cañas, J.E., Long, M., Nations, S., Vadan, R., Dai, L., Luo, M., Ambikapathi, R., Lee, E.H., & Olszyk, D. (2008). Effects of functionalized and nonfunctionalized single-walled carbon nanotubes on root elongation of select crop species. Environmental Toxicology and Chemistry, 27(9), 1922–1931. doi: 10.1897/08-117.1

Clark, E.M.R., Dole, J.M., Carlson, A.S., Moody, E.P., McCall, I.F., Fanelli, F.L., & Fonteno, W.C. (2010). Vase Life ofNew Cut Flower Cultivars. Horttechnology, 20 (6), 1016-1025. doi: https://doi.org/10.21273/HORTSCI.20.6.1016

Dutta, S., & Pati, S.K. (2010). Novel properties of graphene nano ribbons: a review, Journal of Materials Chemistry, 38. doi: https://doi.org/10.1039/C0JM00261E

Geim, A.K., & Novoselov, K.S. (2007).The rise of graphene, Nature Materials, 6, 183-191.

Geisler, M., Li, K., Huang, Y., Chen, Y., Kolmakov, A., & Ma, X. (2013). Phytotoxicity, accumulation and transport of silver nanoparticles by Arabidopsis thaliana. Nanotoxicology, 7(3), 323–337. doi: 10.3109/17435390.2012.658094

Giraldo, J.P., Landry, M.P., Faltermeier, S.M., McNicholas, T.P., Iverson, N.M., Boghossian, A.A., Reuel, N.F., Hilmer, A.J., Sen, F., Brew, J.A., & Strano, M.S. (2014). Plant nanobionics approach to augment photosynthesis and biochemical sensing, Nature Materials. 13, 400–408. doi: 10.1038/nmat3890

Hong, F., Zhou, J., Liu, C., Yang, F., Wu, C., Zheng, L., Yang, P. (2005a). Effect of nano-TiO2 on photochemical reaction of chloroplasts of spinach. Biological Trace Element Research, 105(1–3), 269–279. doi: 10.1385/BTER:105:1-3:269

Husen, A., & Siddiqi. K.S. (2014). Carbon and fullerene nanomaterials in plant system. Journal of Nanobiotechnology , 12, 1–10. doi: https://doi.org/10.1186/1477-3155-12-16

Khodakovskaya, M., Dervishi, E., Mahmood, M., Xu, Y., Li, Z., Watanabe, F., & Biris, A.S. (2009). Carbon nanotubes are able to penetrate plant seed coat and dramatically affect seed germination and plant growth, ACS Nano, 6, 7541. doi: https://doi.org/10.1021/nn900887m

Khodakovskaya, M.V., de, Silva. K., Biris, A.S., Dervishi, E., & Villagarcia, H. (2012). Carbon nanotubes induce growth enhancement of tobacco cells. ACS Nano, 6(3), 2128–2135. doi: https://doi.org/10.1021/nn204643g

Khodakovskaya, M.V., Kim, B.S., Kim, J.N., Alimohammadi, M., Dervishi, E., Mustafa, T., & Cernigla, C.E. (2013). Carbon nanotubes as plant growth regulators: effects on tomato growth, reproductive system, and soil microbial community. Small, 9(1), 115–123. doi: 10.1002/smll.201201225

Kumar, V., Guleria, P., Kumar, V., & Yadav, S.K. (2013). Gold nanoparticle exposure induces growth and yield enhancement in Arabidopsis thaliana. Science of the Total Environment, 461,462–468. doi: 10.1016/j.scitotenv.2013.05.018

Lei, Z., Mingyu, S., Chao, L., Liang, C., Hao, H., Xiao, W., Xiaoqing, L., Fan, Y., Fengqing, G., & Fashui, H. (2007). Effects of nanoanatase TiO2 on photosynthesis of spinach chloroplasts under different light illumination. Biological Trace Element Research, 119, 68–76. doi: 10.1007/s12011-007-0047-3

Lin, D., & Xing, B. (2007). Phytotoxicity of nanoparticles: Inhibition of seed germination and root growth. Environmental Pollution, 150, 243–250. doi: 10.1016/j.envpol.2007.01.016

Liu, C., Li, F., Ma, L.P., Cheng, H.M. (2010). Advanced Materials for Energy Storage Advanced Materials. 22, 28– 62. doi: 10.1002/adma.200903328

Matic, I.Z., Juranic, Z., Savikin, K., Zdunic, G., Nadvinski, N., Godevac, D. (2012). Chamomile and Marigold Tea: Chemical Characterization and Evaluation of Anticancer Activity. Phytotherapy research, 27(6), 852-8. doi:10.1002/ptr.4807

Mohammad, S., Kashani, H.H. (2012) .Pot marigold (Calendula officinalis) medicinal usage and cultivation. Scientific Research and Essays, 7 (14), 1468-1472. doi: 10.5897/SRE11.630

Nair, R., Mohamed, M.S., Gao, W., Maekawa, T., Yoshida, Y., Ajayan, P.M., Kumar, D.S. (2012). Effect of carbon nanomaterials on the germination and growth of rice plants. Journal of Nanoscience and Nanotechnology, 12, 2212–2220. doi: 10.1166/jnn.2012.5775

Prasad, B., & M. K. Sinha. (1981). The relative efficiency of zinc carriers on growth and zinc nutrition of corn. Plant and Soil, 62, 45–52. doi: https://doi.org/10.1007/BF02205024

Preethi, K.C., & Kuttan, R. (2009). Wound healing activity of flower extract of Calendula officinalis. Journal of basic and clinical physiology and pharmacology, 20 (1), 73-79. doi: 10.1515/jbcpp.2009.20.1.73

Qi, M., Liu, Y., Li, T. (2013). Nano-TiO2 improves the photosynthesis of tomato leaves under mild heat stress. Biological Trace Element Research, 156(1–3), 323–328. doi: 10.1007/s12011-013-9833-2

Siddiqui, M.H., Al-Whaibi, M.H. (2014). Role of nano-SiO2 in germination of tomato (Lycopersicum esculentum seeds Mill.), Saudi journal of biological science, 21, 13–17. doi: 10.1016/j.sjbs.2013.04.005

Smirnova, E., Gusev, A., Zaytseva, O., Sheina, O., Tkachev, A., Kuznetsova, E., Lazareva, E., Onishchenko, G., Feofanov, A., & Kirpichnikov, M. (2012). Uptake and accumulation of multiwalled carbon nanotubes change the morphometric and biochemical characteristics of Onobrychis arenaria seedlings. Frontiers of Chemical Science and Engineering, 6.132–138. doi: https://doi.org/10.1007/s11705-012-1290-5

Tiwari, D.K., Dasgupta-Schubert, N., Villaseñor-Cendejas, L.M., Villegas, J., Carreto-Montoya, L., & Borjas-García, S.E. (2014). Interfacing carbon nanotubes (CNT) with plants: Enhancement of growth, water and ionic nutrient uptake in maize (Zea Mays) and implications for nanoagriculture. Applied Nanoscience, 4,577–591. doi: https://doi.org/10.1007/s13204-013-0236-7

Torney, F., Trewyn, B.G., Lin, V.S-Y., & Wang, K. (2007). Mesoporous silica nanoparticles deliver DNA and chemicals into plants. Nature Nanotechnology, 2,295–300. doi: 10.1038/nnano.2007.108

Villagarcia, H., Dervishi, E., Silva, K., Biris, A.S., & Khodakovskaya, M.V. (2012).Surface chemistry of carbon nanotubes impacts the growth and expression of water channel protein in tomato plants. Small, 8, 2328–2334. doi: https://doi.org/10.1002/smll.201102661

Wang, X., Han, H., Liu, X., Gu, X., Chen, K., & Lu, D. (2012a). Multi-walled carbon nanotubes can enhance root elongation of wheat (Triticum aestivum) plants, Journal of Nanoparticle Research, 14(6), 1–10. doi: http://dx.doi. org/10.1155/2014/169245

Warner, R.M., & Erwin, J.E. (2005). Prolonged high temperature exposure and daily light integral impact growth and flowering of five herbaceous ornamental species. Journal of the American Society for Horticultural Science, 130 (3).319-325. doi: 10.21273/JASHS.130.3.319

Xie, Y., Li, B., Zhang, Q., & Zhang, C. (2012).Effects of nano-silicon dioxide on photosynthetic fluorescence characteristics of Indocalamus barbatus McClure. Journal of Nanjing Forest University (Natural Science Edition), 2, 59–63

Xie, Y., Li, B., Zhang, Q., Zhang, C., Lu, K., & Tao, G. (2011).Effects of nano-TiO2 on photosynthetic characteristics of Indocalamus barbatus. Journal of Northeast forestry University, 39, 22–25

Zhang, M., Gao, B., Chen, J., Li, Y. (2015). Effects of graphene on seed germination and seedling growth, Journal of Nanoparticle Research, 17:78

Zheng, L., Hong, F.S., Lu, S.P., Liu, C. (2005). Effect of nano-TiO2 on strength of naturally and growth aged seeds of spinach. Biological Trace Element Research, 104, 83–91. doi: 10.1385/BTER:104:1:083

Zhu, G.C., Xu, Y., Yang, K., Zheng, & X. Sun. (2009). Disk-capped multipod arrays of zinc oxide. Materials Chemistry and Physics, 113, 115–118. doi: https://doi.org/10.1021/ja035569p

Article Statistics

Copyright License

Download Citations

How to Cite

Kunal Adhikary, Tapas Mandal, Jayoti Majumder, & Prasenjit Mandal. (2022). EFFECTS OF GRAPHENE OXIDE NANOSHEET ON GERMINATION AND SEEDLING ATTRIBUTES ON TAGETES ERECTA L. AND CALENDULA OFFICINALIS L. The American Journal of Agriculture and Biomedical Engineering, 4(03), 1–14. https://doi.org/10.37547/tajabe/Volume04Issue03-01