Agriculture and Biomedical
| Open Access |
DOI: Evocation, Regeneration And Delineation, Of A Completely Unique Oxidase From C. Multiflorus ( White Broom )
Garcia Shnyrov , Departamento De Quimica Fisica, Universidad De Salamanca, SpainAbstract
The present work describes a study on the isolation, purification and a few features of a completely unique peroxidase from white Spanish broom, a tree legume very abundant within the northern half Spain and Portugal. Optimal conditions are proposed for enzyme extraction, removal of phenolic compounds and enzyme purification by consecutive hydrophobic, ion-exchange and size-exclusion chromatographies. Peroxidase from white broom was found to possess a relative molecular mass of 49 kDa. The spectrum of CMP showed a Soret band at 403 nm with a Rz factor of three .3. Substrate specificity and therefore the effect of some variables on the activity of CMP with guaiacol as cosubstrate have also been investigated.
Keywords
Oxidase, Plant, Regeneration
References
Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 1976; 72: 248-254.
Cosio C, Dunand C. Specific functions of individual class III peroxidase genes. J. Exp. Bot. 2009; 60: 391-408.4.
Weston LA, Duke SO. Weed and crop allelopathy. Critical Reviews in Plant Sciences. 2003; 22: 367-389.
Rizvi S, Haque H, Singh V, Rizvi V. A discipline called allelopathy Allelopathy: Springer. 1992; 1-10.15.
Romeo JT, Weidenhamer JD. Bioassays for allelopathy in terrestrial plants Methods in Chemical Ecology. Springer. 1998; 2: 179-211.16.
Article Statistics
Copyright License
Copyright (c) 2021 The American Journal of Agriculture and Biomedical Engineering
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors retain the copyright of their manuscripts, and all Open Access articles are disseminated under the terms of the Creative Commons Attribution License 4.0 (CC-BY), which licenses unrestricted use, distribution, and reproduction in any medium, provided that the original work is appropriately cited. The use of general descriptive names, trade names, trademarks, and so forth in this publication, even if not specifically identified, does not imply that these names are not protected by the relevant laws and regulations.