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DOI: Study Of The Effect Of Polysaccharides On Hemostasis
Khoshimov N.N. , Institute of Biophysics and Biochemistry at the National University of Uzbekistan, Uzbekistan Nasirov K.E. , Institute of Biophysics and Biochemistry at the National University of Uzbekistan, Uzbekistan Raimova G.M. , Institute of Biophysics and Biochemistry at the National University of Uzbekistan, Uzbekistan Musaeva M.K. , Institute of Biophysics and Biochemistry at the National University of Uzbekistan, Uzbekistan Azizov V.G. , Namangan State University, Uzbekistan Тuraev A. S. , The Institute of Bioorganic Chemistry named after A.S. Sadikov, Uzbekistan Murоdоv S.S. , Gulistan State University, Uzbekistan Inоyatоv I.I. , Gulistan State University, Uzbekistan Badirdinоv B.R. , Gulistan State University, Uzbekistan Abdusalоmоv Sh.A. , Gulistan State University, UzbekistanAbstract
The effect of sulfated polysaccharides on the hemostatic system in conditions in vitro. Platelet-rich plasma was obtained by centrifugation at 200 g for 10 minutes. The remaining citrate blood was further centrifuged at 1500 g for 10 min to obtain platelet-poor plasma. The antithrombin activity of the compounds was evaluated in vitro by their effect on the recalcification time, thrombin, and prothrombin time of human blood plasma stabilized with a 3.8% sodium citrate solution in the ratio of 9:1. In studies conducted on the blood plasma of rats, it was found that the studied compounds, to varying degrees, lengthen the APTT, APTT, prothrombin time. At the same time, anticoagulant activity was established to block one of the factors II, V, X. Polysaccharide exhibit a combined anticoagulant effect in the body, due to which they are classified as anticoagulant and antithrombin agents.
Keywords
Blood anticoagulants, human plasma, antithrombin
References
Geerts W. Prevention of venous thromboembolism: American College of Chest Physicians evidence-based clinical practice guidelines (8th Edition)/ W. Geerts, D Bergqvist, G.F. Pineo J.A. Heit, C.M. Samama, M.R. Lassen, C.W. Colwell // Chest. - 2008. - 133 (6). - P.381S-453S.
Ageno W. Antithrombotic treatment of splanchnic vein thrombosis: results of an international registry / W Ageno, N Riva, S Schulman, et al. // Semin Thromb Hemost. - 2014.- Feb;40(l). - P.99-105.
Molteni M. Warfarin and atrial fibrillation: from ideal to real the warfarin affaire/ M. Molteni, C. Cimminiello // Thromb J. - 2014. - 12(1). - P.5.; Vo T., 2014.
Vo T. Current state of anticoagulants to treat deep venous thrombosis / T. Vo, S. Vazquez, M.T. Rondina // Curr Cardiol Rep. -2014. -16(3). -P.463.
Марри Р., Греннер Д., Мейес П., Родуэлл В. Биохимия человека / Л. Гинодман. — М: БИНОМ, 2009. — С. 331-336. — 451 с. — ISBN 978-5-9963-0016-7.
Nozim N. Khoshimov Nasirov E. Kabil Kamila A. Eshbakova. Research Influence Biological Active Agents in the Course of Regulation of Functional Activity of Platelets and System of a Haemostasis. European Journal of Medicine Vol. 8, Is. 2, pp. 88-93, 2015 DOI: 10.13187/ejm.2015.8.88
Verhaeghe R., DeMoerloose P., Eikenboom J.C. et al. Genetic and asquired risk factors of venous thromboembolism. In: Pulmonary Vascular Pathology: A Clinical Update. Eds. M. Demedts, M. Delcroix, R. Verhaeghe, G.M. Verleden. EurRespSoc 2004. –С. 9.
Nasirov K.E., Musaeva M.K., Khoshimov N.N., Raimova G.M., Turaev A.S., Muhitdinov B.I. Influence of some sulphated polysaccharides on the platelet aggregation in normal and in patients with ischemic heart disease. International Journal of Psychosocial Rehabilitation. Volume 24 - Issue 8. Pages: 6976-6985. DOI: 10.37200/IJPR/V24I8/PR280715
Nadjimova, X. K., Khoshimov, N. N., Muhitdinov, B. I., & Musayeva, M. K. (2006). FIBRINOLYTIC ACTIVITY OF POLYSACCHARIDES WITH ANTICOAGULANT PROPERTIES. O ‘ZBEKISTON BIOLOGIYA JURNALI, 8.
Wang Z.M., Li L., Zheng B.S., Normakhamatov N., GuoS.Y. Preparation and anticoagulation activity of sodium cellulose sulfate // Int. J. Biol. Macromol. 2007. Vol.41 (2). -P. 376-382.
Huynh R., Chaubet F., Jozefonvicz J. Anticoagulant properties of dextranmethylcarboxylate benzylamide sulfate (DMCBSu); a new generation of bioactive functionalized dextran // Carbohydr. Res. 2001. Vol. 332. -P. 75-83. 10.
Mahner C., Lechner M. D., Nordmeier E. Synthesis and characterization of dextran and pullulan sulphate // Carbohydr. Res. 2001. Vol. 331. -P. 203-208.
Scaglione F. 2013. New oral anticoagulants: comparative pharmacology with vitamin K antagonists. ClinPharmacokinet. - 522: 69-82. doi: 10.1007/s40262-012-0030-9.
Laboratory research methods in the clinic. 1987. Reference. Ed. V.V. Menshikov. M.: Medicine: 172.
Teien, A.N., Lie M. 1975. Heparin assay in plasma a comparison of five clotting methods. Thromb Res.-V.7. -N5: 777 - 788. doi.org/10.1016/0049-3848(75)90202-9.
Yin E.T. Wessler S., J. Butler. 1973. Plasma heparin: a unique, practical, submicrogram-sensitive assay. J Lab Clin Med. - V. 81. - N 2: 298-310.
Muhitdinov B, Heinze T, Normakhamatov N, Turaev A. 2017. Preparation of sodium cellulose sulfate oligomers by free-radical depolymerization. Carbohydr Polym [Internet]. 173:631–7. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0144861717306707.
Muhitdinov B, Heinze T, Turaev A, Koschella A, Normakhamatov N. 2019. Homogenous synthesis of sodium cellulose sulfates with regulable low and high degree of substitutions with SO3/Py in N,N-dimethylacetamide/LiCl. Eur Polym J [Internet]. 119:181–8. Available from: https://linkinghub.elsevier.com/retrieve/pii/S0014305719312558
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