Enhancing Fruit Growth of Daisy Mandarin (Citrus Reticulata) Through Foliar Application of Manganese and Iron
Bindia , Department of Horticulture, Faculty of Agriculture, Guru Kashi University, Talwandi Sabo (Punjab), 151302 Dr. Navdeep Singh , Department of Horticulture, Faculty of Agriculture, Guru Kashi University, Talwandi Sabo (Punjab), 151302 Mr. Gurdeep Singh , Department of Horticulture, Faculty of Agriculture, Guru Kashi University, Talwandi Sabo (Punjab), 151302 Dr. M.K. Kaul , Department of Horticulture, Faculty of Agriculture, Guru Kashi University, Talwandi Sabo (Punjab), 151302Abstract
The study titled “Impact of Micronutrients (Mn and Fe) on Vegetative Growth of Daisy Mandarin (Citrus reticulata)” was carried out during 2024–25 at Guru Kashi University, Talwandi Sabo, Punjab. The research aimed to assess the effects of foliar applications of manganese (Mn) and iron (Fe) on the vegetative growth, fruit physical attributes, chemical composition, and overall quality of Daisy mandarin. Manganese sulphate (MnSO₄) and ferrous sulphate (FeSO₄) were applied at concentrations of 0.1%, 0.2%, and 0.3%, both individually and in combination. The experiment followed a Randomized Block Design, comprising sixteen treatments with four replications, resulting in sixty-four treatment combinations. It was conducted in a six-year-old Daisy mandarin orchard budded on Jatti Khatti (Citrus jambhiri Lush) rootstock. Key parameters measured included scion girth, rootstock girth, and canopy volume. The treatment combining 0.3% MnSO₄ and 0.2% FeSO₄ yielded the most significant improvements in vegetative parameters, with a scion girth of 10.20 cm, rootstock girth of 9.8 cm, and canopy volumes of 134.0 m³ (north-south) and 115.0 m³ (east-west), compared to the control. These findings demonstrate that foliar application of MnSO₄ and FeSO₄, across various combinations, markedly enhanced the vegetative growth of Daisy mandarin under subtropical conditions. The study underscores the importance of micronutrient supplementation in improving orchard productivity and provides valuable insights for subtropical horticulture practices.
Keywords
Daisy mandarin, Citrus reticulata, manganese sulphate, ferrous sulphate, foliar application, micronutrients, vegetative growth, subtropical horticulture, Randomized Block Design, Jatti Khatti rootstock
References
Alonso, J., et al. (2023). Global citrus production trends. Journal of Horticultural Science, 45(3), 123–134.
Abd-Allah, A.S. (2006). Effect of spraying some macro and micro nutrients on fruit set, yield and fruit quality of Washington Navel orange trees. Journal of Applied Sciences Research, 2: 1059–1063.
Al-Obeeda, R.S., Mahmoud, A.A., Hassan, A., & Saifa, A.M. (2017). Improvement of Kinnow mandarin fruit productivity and quality by urea, boron and zinc foliar spray. Journal of Plant Nutrition, 1–10.
Anonymous. (2018b). Area and Production of Horticulture Crops in India, 2017–2018. Government of India; National Horticulture Board, Ministry of Agriculture and Farmers Welfare.
Anonymous. (2021). Package of Practices for Fruit Crops (pp. 1–2). Ludhiana, Punjab: Punjab Agricultural University.
Arora, V., Sharma, S., Singh, M., Gupta, H.S., Rattanpal, H.S., & Kaur, K. (2024a). Characterization of healthy and split Daisy Mandarin (Citrus reticulata Burm.) fruits from a physiological and biochemical standpoint. New Zealand Journal of Crop and Horticultural Science, 1–18. https://doi.org/10.1080/01140671.2024.2371970
Babu, K.D., Dubey, A.K., & Yadav, D.S. (2007). Effect of micronutrients on enhancing the productivity and quality of Kinnow mandarin. Indian Horticultural Journal, 64(3), 353–356.
Boaretto, A.E., Boaretto, R.M., Muraoka, T., et al. (2002). Foliar micronutrient application effects on citrus fruit yield, soil and leaf Zn concentrations, and 65Zn mobilization within the plant. Acta Horticulturae.
El-Saida, S.A.G. (2001). Effect of some growth regulators and zinc sulphate treatments on yield and quality of Washington Navel orange. Annals of Agricultural Science, 39, 1199–1212.
FAO. (2023). Food and Agriculture Organization of the United Nations [Internet]. Available from: http://www.fao.org/faostat/en/#data [Accessed: June 2, 2023].
Gill, M.S., Gill, P.P.S., Brar, J.S. and Khehra, S. (2017). Daisy Mandarin performance in the subtropics of northwest India on three distinct rootstocks. Conservation of the Environment and Ecology, 23, 177–183.
Gomez, K. A., & Gomez, A. A. (1983). Statistical Procedures for Agricultural Research. John Wiley & Sons.
Gullo, G., et al. (2023). Fruit quality assessment based on mineral elements and juice properties in nine citrus cultivars. Frontiers in Plant Science, 14, 1032456.
Gurjar, M.K., Kaushik, R.A. and Baraily, P. (2015). Effect of zinc and boron on the growth and yield of Kinnow mandarin. International Journal of Scientific Research, 4, 207–208.
Ingle, H.V., Kokate, S.S., Athawale, R.B. and Katole, S.R. (2002). Effect of foliar application of zinc and iron on growth, yield and quality of acid lime. Indian Journal of Citriculture, 1(1), 43–45.
Kaur, K., Chahal, T.S., Singh, G., Gupta, M. and Rattanpal, H.S. (2024b). Effects of foliar growth regulator application on daisy mandarin (Citrus reticulata) fruit splitting, yield, and quality. Indian Journal of Agricultural Sciences, 94(2), 181–186. https://doi.org/10.56093/ijas.v94i2.143183
Kaur, N., et al. (2024a). Daisy mandarin: Agronomic and nutritional traits. HortScience, 59(6), 789–797.
Khan, N., Khan, R.A., Farid, G., Ashraf, M.Y., Saleem, M., Shafiq, F., Anwar, S. and Ashraf, M.Y. (2022). Citrus reticulata quality features and fruit retention are enhanced by integrated hormonal and nutritional management. Journal of Plant Nutrition, 46, 83–100.
Khurshid, F., Khattak, R.A. and Sarwar, S. (2008). Effect of foliar-applied (Zn, Fe, Cu & Mn) in citrus production. Science Technology & Development, 27(1–2), 34–42.
Lado, J., et al. (2024). Key determinants of citrus fruit quality: Metabolites and main changes during maturation. Scientia Horticulturae, 319, 112134.
Madarakhandi, S., Patil, D.R., Shafeeq, L.B. and Swamy, G.S.K. (2014). Studies on the influence of micronutrient on growth, yield and quality of Kinnow mandarin. Trends in Biosciences, 7(9), 786–788.
Meena, K., Yadav, P.K., Singh, R.S. and Sharma, B.D. (2009). Effect of foliar spray of micronutrients status, yield and fruit quality of Kinnow mandarin in Haryana. Journal of Horticultural Science, 38(3/4), 200–202.
Nanaya, K.A., Anjaneylu, K. and Kotur, S.C. (1985). Effect of foliar applied Zn, Mn, Cu and Mg on growth parameters, chlorosis and interrelationships of micronutrients in leaf tissue of Coorg mandarin. Progressive Horticulture, 17, 309–314.
Perveen, S., and Rehman, H. (2000). Effect of foliar application of zinc, manganese and boron in combination with urea on the yield of sweet orange. Pakistan Journal of Agricultural Research, 16(2), 135–141.
Ram, R.A. and Bose, T.K. (2000). Effect of foliar application of magnesium and micronutrients on growth, yield and fruit quality of mandarin orange (Citrus reticulata Blanco). Indian Journal of Horticulture, 57(3), 215–220.
Razeto, B. and Salas, A. (1986). Magnesium, manganese, and zinc sprays on orange trees (Citrus sinensis (L.) Osbeck). SpringerLink.
Razzaq, K., Khan, A.S., Malik, A.U., Shahid, M. and Ulla, S. (2013). Foliar application of zinc influences the leaf mineral status, vegetative and reproductive growth, yield and fruit quality of Kinnow mandarin. Journal of Plant Nutrition, 36, 1479–1495.
Sharma, R., et al. (2024). Nutritional benefits of citrus flavonoids. Journal of Food Biochemistry, 46(1), e13987.
Singh, D., Mirza, A., Kumar, A., Singh, R., Pratap, S. and Singh, B. (2017). Impact of zinc and boron on growth, yield and quality of Kinnow (Citrus deliciosa × Citrus nobilis) in sub-tropical conditions of Punjab. Journal of Pure and Applied Microbiology, 11, 1135–1139.
Talon, M., Caruso, M. and Gmitter, F.G. (Eds.) (2020). The Genus Citrus (p. 538). Duxford: Elsevier. https://doi.org/10.1016/C2016-0-02375-6
Tariq, M., et al. (2024). Micronutrient management in citrus crops. Agricultural Reviews, 39(2), 87–95.
Tariq, M., Sharif, M., Shah, Z., and Khan, R. (2007). Effect of foliar application of micronutrients on the yield and quality of sweet orange (Citrus sinensis L.). Pakistan Journal of Biological Sciences, 10(11), 1823–1828.
Vijaya, H.M., Godara, R.K., Singh, S., and Sharma, N. (2017). Effect of exogenous application of micronutrients on growth and yield of Kinnow mandarin under semi-arid zone of Haryana. Journal of Pharmacognosy and Phytochemistry, 6(4), 733–735.
Zhong, S., Jin, H., Wang, Y. and Jiao, Y. (2023). Evaluation of fruit quality in nine citrus varieties based on juice characteristics and mineral components. [Journal unspecified].
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