The nitrogen transporters (NRT) play an essential role in the uptake, translocation, and regulation of nitrogen, which is a key macronutrient for plants. The identification of NRT gene families and their expression patterns under stress conditions provides insights into how plants adapt to nutrient deficiencies. In this study, we identify and characterize the NRT gene family in grapevine (Vitis vinifera) and analyze their expression profiles in leaves under nitrogen-deficiency stress. A comprehensive bioinformatics approach was used to identify 15 putative NRT genes in the grape genome. Expression analysis under nitrogen-deficiency stress was carried out using quantitative PCR (qPCR) in grape leaves. Results suggest that certain NRT genes are upregulated in response to nitrogen-deficiency stress, highlighting their role in nitrogen uptake and transport. These findings contribute to understanding the molecular mechanisms of nitrogen stress tolerance in grapevines and could guide future breeding strategies for improving nitrogen use efficiency in crops.