Applied Sciences
| Open Access |
DOI: OPTIMIZATION OF ACTINOKINASE SYNTHESIS FROM LOCAL ISOLATE USING RESPONSE SURFACE METHODOLOGY: OPTIMIZATION OF PHYSICAL CONDITIONS
Mawada Elimam Ibrahim , Faculty of Agriculture, University of Khartoum, Khartoum, SudanAbstract
This study aims to optimize the synthesis of actinokinase from a local isolate by focusing on the optimization of physical conditions using Response Surface Methodology (RSM). Actinokinase is an enzyme with significant potential in various industries, including pharmaceuticals and biotechnology. The physical conditions, including temperature, pH, and agitation rate, are critical factors influencing actinokinase production. RSM, a statistical tool, allows for the simultaneous optimization of multiple variables, enabling the determination of the optimal physical conditions for actinokinase synthesis. By employing RSM, the study identifies the optimal combination of physical conditions that maximize actinokinase production, enhancing its potential applications.
Keywords
synthesis optimization, local isolate, physical conditions
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