Articles
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DOI: CAENORHABDITIS ELEGANS INFRARED-BASED MOTILITY ASSAY: IDENTIFYING NEW HITS FOR NEMATICIDE DRUG DEVELOPMENT
Gonzalo Salinas , Worm Biology Laboratory, Institut Pasteur De Montevideo, Montevideo, UruguayAbstract
The identification of effective nematicide drugs is crucial for the management of plant-parasitic nematodes that pose significant threats to agriculture. In this study, we employed a Caenorhabditis elegans (C. elegans) infrared-based motility assay as a high-throughput screening platform to identify potential hits for nematicide drug development. The assay utilized automated tracking and analysis of C. elegans movement patterns in response to chemical compounds. A library of diverse compounds was screened, and the effects on C. elegans motility were assessed. Several compounds demonstrated significant inhibition of C. elegans movement, indicating their potential as nematicides. These hits were further characterized for their selectivity and efficacy against different stages of nematode development. Overall, this study highlights the utility of the C. elegans infrared-based motility assay in identifying new candidates for nematicide drug development.
Keywords
Caenorhabditis elegans, nematicide, drug development
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