This research paper focuses on the development and validation of an RP-HPLC (Reverse-Phase High-Performance Liquid Chromatography) method for the simultaneous estimation of cobicistat and atazanavir in bulk and pharmaceutical dosage form. Cobicistat and atazanavir are important antiretroviral drugs used in the treatment of HIV infection. The development of a reliable and accurate analytical method for their simultaneous determination is essential for quality control and pharmacokinetic studies. The RP-HPLC method was optimized by considering factors such as column selection, mobile phase composition, flow rate, and detection wavelength. Validation parameters including linearity, precision, accuracy, specificity, robustness, and system suitability were evaluated according to international guidelines. The developed method demonstrated good linearity, sensitivity, and selectivity for the simultaneous estimation of cobicistat and atazanavir in both bulk and pharmaceutical dosage form. The findings of this study provide a robust and reliable analytical method that can be utilized for routine analysis and quality control of cobicistat and atazanavir formulations.

One of the frequent complications of diabetes mellitus (DM) is the lesion of human blood vessels, leading to the development of diabetic foot. Literature and daily practical observation show an increase of the rate of diabetic foot (30-70%), which raises the actuality of the problem of diabetic foot treatment. This article contains data concerning the results of surgical treatment of 151 patients with diabetes mellitus complicated by diabetic foot and purulent-necrotic soft tissue inflammation. Our work presents the basic principles of treatment of purulent-necrotic complications of diabetes mellitus. The effectiveness of local application of Acerbin solution and two-stage surgical tactics in the complex surgical treatment of purulent-necrotic complications of diabetes mellitus has been proved.

Antibiotic drug resistance has emerged as a critical global challenge, posing a substantial threat to public health, medical treatments, and healthcare systems worldwide. This paper delves into the multifaceted dimensions of antibiotic drug resistance, encompassing its underlying mechanisms, the factors driving its spread, and the far-reaching consequences it engenders. By analyzing the intricate interplay between microbial evolution, inappropriate antibiotic usage, and the lack of novel drug development, this study aims to shed light on the urgency of addressing this crisis. Furthermore, potential strategies and interventions to mitigate antibiotic drug resistance are explored, emphasizing the collaborative efforts required across medical, governmental, and societal domains. The paper underscores the need for a comprehensive and sustained approach to combat this global challenge, safeguarding the effectiveness of antibiotics for current and future generations.

The article presents a comparative analysis of the effectiveness of traditional and interactive teaching methods in postgraduate education in dentistry. A comparative evaluation of the results of the effectiveness of traditional and interactive methods of teaching has been carried out by questionnaire on targeted questions of cadets and faculty members who participated in the Cycle of general improvement in dentistry: the interactive method is proved to be more promising than the traditional system of education in the professional formation of dentists.