This study evaluated the potential of Acalypha wilkesiana leaf powder (AWLP) to mitigate the toxic effects of Aflatoxin B₁ (AFB₁) in broiler chickens. A 3×3 factorial design was used to assess the interaction between three levels of AFB1 (0, 0.25, and 0.5 mg/kg) and three levels of AWLP (0, 250, and 500 mg/kg) in the diets of Cobb 500 broilers. One hundred- and eighty-nine-day-old chicks were randomly assigned to nine dietary treatments over a 6-week feeding trial. Oxidative stress biomarkers and cellular stress markers were evaluated. AFB₁ exposure significantly induced oxidative damage in brain and muscle tissues, as reflected by elevated lipid peroxidation and reduced antioxidant enzyme activities (catalase and glutathione peroxidase). AWLP supplementation at 250 mg/kg reduced lipid peroxidation, and significant interaction effects were observed for several parameters, including NF-κB, HSP70, and oxidative stress markers, confirming AWLP’s dose-dependent modulatory role. Although AWLP demonstrated antioxidant and hepatoprotective benefits, it was insufficient as a standalone remedy for AFB₁ toxicity. These findings suggest that AWLP at moderate levels may serve as a functional phytogenic additive in poultry feed, particularly in regions prone to aflatoxin contamination, but further integration with other detoxifiers is recommended.

Acalypha wilkesiana, Aflatoxin B1, broiler, oxidative stress, phytogenic additives, cellular biomarkers, poultry health

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