The monograph examines an original methodology for optimizing the long-distance transport of live honey-bee colonies, developed from hands-on experience with U.S. pollination services. The topic’s relevance stems from agriculture’s growing reliance on honey-bee pollination and the attendant logistical challenges. Yet transporting bees carries a high risk of stress and mortality due to extremes of temperature, vibration, prolonged overheating, and other factors. Traditional transport practices are often piecemeal and based on empirical rules, resulting in substantial losses—up to 30–40 percent of the bees under adverse conditions—and degraded pollination quality.