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DOI: Characterization Of A Bacterium – Producer Of Amidase
Lobar Khasanova , Researcher, National University Of Uzbekistan, Tashkent, UzbekistanAbstract
We analyzed 11 samples of wastewater, sludge and soil contaminated with acrylic acid nitrile, acrylamide and acrylic acid from the territory of Navoiyazot JSC (Navoi, Uzbekistan). As a result, 19 strains belonging to the bacteria of the genus Rhodococcus were isolated into a pure culture. In the process of selection, 2 strains of bacteria were selected that exhibit the highest amidase activity for acrylic acid (0.712 U / mg and 0.868 U / mg). Strains of Rhodococcus sp. - 8/4/1 and Rhodococcus sp. - 3/4/3 are potential amidase producers.
Keywords
Strain, Acrylic Acid, Producer, Amidase, Enzyme Activity
References
Ivshina, I.B. The genus Rhodococcus bacteria: biodiversity, detection, immunoassay. Doctoral thesis. Perm, 1997. (In Russ.).
Ivshina, I.B., Kamenskikh, T.N., Anokhin, B.A. Adaptive mechanisms of alkanotrophic rhodococci survival under unfavorable conditions. Bulletin of Perm University, V. 5 (10) (2007). Р. 107-112. (In Russ.).
Kim D., Choi K.Y., Yoo M., Zylstra G.J., Kim E. Biotechnological potential for biodegradation of Rhodococcus. J. Microbiol. Biotechnol.2018 V.28 (7). P. 1037-1051
Alvarez, H.M. Biology of Rhodococcus, 2nd ed.; Springer: Basel, Switzerland, 2019.
Larkin, M.J.; Kulakov, L.A.; Allen, C.C.R. Biodegradation by Members of the Genus Rhodococcus: Biochemistry, Physiology, and Genetic Adaptation. Adv. Appl. Microbiol. 2006, 59, 1–29. [PubMed]
Zampolli, J.; Zeaiter, Z.; Di Canito, A.; Di Gennaro, P. Genome analysis and -omics approaches provide new insights into the biodegradation potential of Rhodococcus. Appl. Microbiol. Biotechnol. 2019, 103, 1069–1080. [CrossRef] [PubMed]
Abdelraheem, E.M.M.; Busch, H.; Hanefeld, U.; Tonin, F. Biocatalysis explained: From Pharmaceutical to Bulk Chemical Production. React. Chem. Eng. 2019. [CrossRef]
Gröger, H.; Asano, Y.; Bornscheuer, U.T.; Ogawa, J. Development of biocatalytic processes in Japan and Germany: From research synergies to industrial applications. Chem. Asian J. 2012, 7, 1138–1153. [CrossRef] [PubMed]
Ogawa, J.; Shimizu, S. Industrial microbial enzymes: Their discovery by screening and use in large-scale production of useful chemicals in Japan. Curr. Opin. Biotechnol. 2002, 13, 367–375. [CrossRef]
Neerja Thakur, Vijay Kumar, Nirmal Kant Sharma, Shikha Thakur, Tek Chand Bhalla. Aliphatic Amidase of RhodococcusRhodochrous PA-34: Purification, Characterization and Application in Synthesis of Acrylic Acid Protein Pept Lett 2016;23(2):152-8.
Nesterenko O.A., E.I. Krasnikov, T.M. Nogina. Nocardi-like nocardioform bacteria - Kiev: Naukova Dumka - 1985 - P. 336.
O.A. Gimadutdinov, A.R. Kayumov Workshop on Molecular Genetics. Study guide. Kazan, KFU (2016), p. 36.
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