Our study introduces a real-time, non-destructive strategy for monitoring self-healing in biocement by integrating genetically engineered bioluminescent microorganisms. Microcracking in concrete infrastructure imposes annual repair expenditures exceeding US$18 billion in the United States, underscoring the need for effective in-situ diagnostics. Although microbially induced calcium carbonate precipitation (MICP) offers an attractive self-healing mechanism, existing evaluation techniques are invasive, intermittent, and incapable of capturing healing kinetics. We engineered three bacterial strains— Sporosarcina pasteurii, Bacillus subtilis, and Pseudomonas aeruginosa—to constitutively express luciferase, enabling emission of quantifiable light signals proportional to metabolic activity during mineralisation. Laboratory experiments across diverse environmental conditions and encapsulation schemes revealed a robust correlation (R² = 0.92) between bioluminescence intensity and calcium carbonate precipitation rate, with microcracks as small as 10 µm reliably detected. Field-scale validation under simulated climatic cycles confirmed sustained signal integrity over 24 monitoring events during twelve months, while achieving crack-closure efficiencies between 75 % and 89 %. The proposed biosensing platform furnishes unprecedented insight into temporal healing dynamics, facilitating optimisation of microbial formulations, predictive maintenance scheduling, and deeper elucidation of microbe–mineral interactions in cementitious matrices. Its implementation could significantly extend service life and reduce lifecycle costs of critical infrastructure assets. Beyond concrete, the technology can be adapted to other structural materials where real-time, autonomous health monitoring is imperative.

Biocement, self-healing concrete, bioluminescent microbes, real-time monitoring, microbially induced calcium carbonate precipitation, luciferase, non- destructive testing, sustainable infrastructure

McCallister, E. (2025). Evaluating the aviation infrastructure in West Virginia as part of the American Society of Civil Engineers (ASCE) Infrastructure Report Card. Proceedings of the West Virginia Academy of Science, 97(2). https://doi.org/10.55632/pwvas.v97i2.1119.

Barrias, A., Casas, J. R., C Villalba, S. (2023). Development of optical fiber sensors for chloride ion detection in reinforced concrete structures. Structural Health Monitoring, 22(3), 1258-1273.

Tiejun Liu, Yangyu Fu, Kexuan Li, Ao Zhou, Renyuan Qin, Dujian Zou, Experimental investigation and theoretical analysis of long-term performance for optical fiber Bragg grating-fiber reinforced composite in alkaline concrete environment, Case Studies in Construction Materials, Volume 22, 2025, e04130, ISSN 2214-5095, https://doi.org/10.1016/j.cscm.2024.e04130.

de Belie, N., Gruyaert, E., C Al-Tabbaa, A. (2023). A comprehensive review of autogenous healing in cementitious materials: Mechanisms, influencing factors, and enhancement techniques. Cement and Concrete Research, 163, 106810.

Dong, B., Wang, Y., C Fang, G. (2024). Recent trends in autonomic healing of concrete: From microcapsules to vascular networks. Construction and Building Materials, 365, 129950.

Gutiérrez, J. C., Amaro, F., C Díaz, S. (2022). Applications of bacterial bioluminescence in environmental monitoring: From laboratory to field implementations. Reviews in Environmental Science and Bio/Technology, 21(1), 133-156.

Harle, S.M. Machine learning algorithms on self-healing concrete. Asian J Civ Eng 26, 1381–1394 (2025). https://doi.org/10.1007/s42107-025-01272-4.

Khaliq, W., C Ehsan, M. B. (2022). Crack healing in concrete using various bio influenced self-healing techniques. Construction and Building Materials, 249, 118782.

Kotlobay, A. A., Dubinnyi, M. A., C Yampolsky, I. V. (2023). Emerging trends in bioluminescence engineering: Novel luciferase systems and their applications. Chemical Reviews, 123(5), 2932-3001.

Li, G., Jiang, B., C Zhao, L. (2023). Evaluation methods for self-healing efficiency in cementitious materials: A comparative review. Construction and Building Materials, 353, 129452.

Lin, Y., Zhang, H., C Wang, S. (2024). Light-emitting concrete incorporating bioluminescent algae for sustainable architectural applications. Journal of Cleaner Production, 387, 135666.

Liu, X., Wong, L., C Tan, M. H. Y. (2024). Advanced optical fiber sensors for moisture detection in concrete structures. Sensors, 24(2), 465.

Nguyen, T. H., Kim, J. H., C Lee, H. K. (2023). Bioluminescent bacteria as biological indicators for moisture penetration in building materials. Scientific Reports, 13(1), 1-12.

Rodriguez-Navarro, C., Ruiz-Agudo, E., C Burgos-Cara, A. (2023). Crystalline biomineral formation by bacteria: Polymorphism, nucleation, and mineral-microbe interactions. American Mineralogist, 108(4), 665-683.

Sánchez, M., Faria, P., C Ferrara, L. (2023). Environmental scanning electron microscopy observations of biomineralization processes in concrete: Methodological approaches and insights. Micron, 165, 103311.

Seifan, M., C Berenjian, A. (2023). Microbially induced calcium carbonate precipitation for self-healing concrete applications: From fundamentals to field implementation. Chemical Engineering Journal, 453, 139871.

Silva, F. B., De Belie, N., C Boon, N. (2024). Multi-species bacterial communities for enhanced self-healing capacity in concrete. Construction and Building Materials, 366, 129982.

Wang, J. Y., De Belie, N., C Verstraete, W. (2022). Ecological and economic assessment of different microbially induced carbonate precipitation pathways for self-healing concrete. Journal of Cleaner Production, 331, 129949.

Wang, K., Sun, Q., C Zhang, L. (2024). Time-lapse X-ray computed tomography visualization of self-healing processes in concrete: Insights from 4D imaging. Cement and Concrete Research, 167, 106941.

Wei, S., Wang, S., C Zhang, M. (2023). Recent advances in urease-based MICP for biocementation: Mechanisms, applications, and future perspectives. Biotechnology Advances, 60, 107998.

Wong, L., Liu, X., C Tan, M. H. Y. (2023). Distributed fiber optic sensing for strain monitoring in a concrete dam: Installation techniques and long-term performance. Structural Health Monitoring, 22(1), 252-268.

Zhang, J., Liu, Y., C Feng, T. (2023). Microbially induced calcium carbonate precipitation using Sporosarcina pasteurii for self-healing concrete applications: Optimization and field implementation. Construction and Building Materials, 359, 129568.

Zhang, L., Wang, K., C Deng, M. (2024). Optical fiber-based fluorescence sensing in cement-based composites: From lab-scale experiments to field applications. Cement and Concrete Composites, 145, 104923.

Zhang, Y., Zhao, J., C Tian, H. (2024). Mechanical recovery assessment of self-healing concrete: A critical review of testing methods and performance evaluation criteria. Engineering Fracture Mechanics, 283, 108760.