Diagnosing Disease Of Cow Mastitis And Method Of Using Diffusion-Precipitation Reaction In Agar Gel

The microbial factor is essential in the etiology of mastitis. In this regard, bacteriological diagnostics is one of the decisive moments in the recognition and differentiation of pathological conditions of the mammary gland. However, it takes a lot of time to make a diagnosis using general methods, special culture media and reagents.


INTRODUCTION
The role of microorganisms in the etiology of mastitis is important, and bacteriological diagnosis is one of the decisive factors in the pathological process in the mammary gland and its comparison.
Diagnosis with commonly used methods requires a certain amount of time, special nutrient media, and reagents to separate the pure culture from the milk and differentiate it.

MATERIAL AND METHODS
The aim of our study was to save time in the process of improving bacteriological diagnostics, to isolate the pathogen, to determine its pathogenicity, not to reduce the pathogenicity of the pathogen used in previously available methods.
The main focus is on staphylococci and streptococci, which are the main causative agents of mastitis, and in our investigations we focused on the diffuse precipitation reaction in agar.We performed the reaction according to the Oudin and Ouxterloni method.
Such a choice is based on a rapid diagnosis of infectious diseases, based on the literature.
On the other hand, it is important in the diagnosis of mastitis.We modified the Sadjiyama, Bergdola, and Dhaka methods in diagnosing mastitis.Pathogenic staphylococci present in milk can be found in mastitis and do not require isolation of pure culture.
This involves inoculating the sample with a mixture of milk and antitoxic whey.
The pathogenicity of staphylococci can be determined by the production of a toxin (antigen).
Staphylococci react with antitoxic serum to form a precipitate line.

The following elements were reworked in the reaction modification:
1) Method of sowing a milk sample 2) The amount of sowing 3) Interval of sowing line with antitoxic staphylococcal serum, staphylococcus.

RESULTS OF THE RESEARCH
The results of the study showed that the sowing of milk is carried out in a straight line or with a bar length of 2-3 cm in the amount of 0.01 cm3, the distance from the pit to the whey is 0.8-1cm.
During the inspection, 151 milk samples from healthy and mastitis-infected animals were examined, of which 58 samples were isolated from staphylococcal cultures.
Toxicity was detected in 58% of isolated cultures even when the DPR method was compared with the classical method.The results obtained are very consistent with plasmocoagulase and hemolysis, DNAmourning reactions, which came close to 61.65.66%.In addition, the examination time was 3-4 days, while the DPR reaction was 2 days.
The sensitivity of the method was 10 microbial bodies per 1 cm3.
DPR is aimed at bacteriological diagnosis and serological types of mastitis caused by streptococci.The Kunter method caught our attention from pre-existing modifications.Its implementation is very convenient and easy, the results are clear, it is convenient to put the precipitation lines, NaCl was added to Difko agar and the spacing of the pits was 4-6 mm.
In parallel, 25 isolated cultures were examined based on Lansfield's precipitation reaction.We used polysaccharide C as the antigen of the reaction.
We prepared the antigen for the Lensfield reaction in an acidic manner.For the DPR reaction, the antigen was prepared by Kunter's express method by precipitation of a culture of staphylococci in a saline solution, autoclaved, and precipitated.
The American Journal of Veterinary Sciences and Wildlife Discovery (ISSN-2689-0968)