https://theamericanjournals.com/index.php/tajas <p>E-ISSN <strong>2689-0992</strong></p> <p>DOI Prefix <strong>10.37547/tajas</strong></p> <p>Started Year <strong>2019</strong></p> <p>Frequency <strong>Monthly</strong></p> <p>Language <strong>English</strong></p> <p>APC <strong>$450</strong></p> The USA Journals en-US The American Journal of Applied Sciences 2689-0992 <p><em>Authors retain the copyright of their manuscripts, and all Open Access articles are disseminated under the terms of the <a href="https://creativecommons.org/licenses/by/4.0/"><strong>Creative Commons Attribution License 4.0 (CC-BY)</strong></a>, which licenses unrestricted use, distribution, and reproduction in any medium, provided that the original work is appropriately cited. The use of general descriptive names, trade names, trademarks, and so forth in this publication, even if not specifically identified, does not imply that these names are not protected by the relevant laws and regulations.</em></p> https://theamericanjournals.com/index.php/tajas/article/view/6324 <p>Gram staining is a cornerstone technique in microbiology for the preliminary identification of bacteria, differentiating them into Gram-positive and Gram-negative groups based on cell wall composition [14]. Traditionally, crystal violet serves as the primary stain; however, concerns regarding its potential toxicity and environmental impact have spurred interest in natural, eco-friendly alternatives [12]. This study investigates the potential of <em>Nephelium lappaceum</em> (rambutan) peel extract, rich in anthocyanins, as a novel primary stain for Gram staining. Through an observational assessment, the study aims to evaluate its staining efficacy, color characteristics, and differentiation capabilities compared to conventional crystal violet. The methods would involve preparing the extract, applying it in a modified Gram staining procedure to representative bacterial cultures, and evaluating the results microscopically. Preliminary observations suggest that rambutan peel extract exhibits promising staining properties, effectively differentiating bacterial cell types. The findings highlight the potential of this natural extract as a sustainable and safer alternative, contributing to greener laboratory practices in bacterial identification.</p> Dr. Nurul Afiqah Binti Razak Dr. Maria Kristina D. Santos Copyright (c) 2025 Dr. Nurul Afiqah Binti Razak, Dr. Maria Kristina D. Santos https://creativecommons.org/licenses/by/4.0 2025-07-01 2025-07-01 7 07 1 7